Extremely LF EMF exposure and restraint stress induce changes on the brain .....
dinsdag, 29 mei 2018 - Categorie: Onderzoeken
BMC Neurosci. 2018 May 21;19(1):31. doi: 10.1186/s12868-018-0432-1.
Extremely low frequency electromagnetic field exposure and restraint stress induce changes on the brain lipid profile of Wistar rats.
Martínez-Sámano J1, Flores-Poblano A1, Verdugo-Díaz L2, Juárez-Oropeza MA1, Torres-Durán PV3.
1 Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Escolar s/n, Ciudad Universitaria, C.P. 04510, Mexico City, Mexico.
2 Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Escolar s/n, Ciudad Universitaria, C.P. 04510, Mexico City, Mexico.
3 Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Escolar s/n, Ciudad Universitaria, C.P. 04510, Mexico City, Mexico. firstname.lastname@example.org.
Exposure to electromagnetic fields can affect human health, damaging tissues and cell homeostasis. Stress modulates neuronal responses and composition of brain lipids. The aim of this study was to evaluate the effects of chronic extremely low frequency electromagnetic field (ELF-EMF) exposure, restraint stress (RS) or both (RS + ELF-EMF) on lipid profile and lipid peroxidation in Wistar rat brain.
Twenty-four young male Wistar rats were allocated into four groups: control, RS, ELF-EMF exposure, and RS + ELF-EMF for 21 days. After treatment, rats were euthanized, the blood was obtained for quantitate plasma corticosterone concentration and their brains were dissected in cortex, cerebellum and subcortical structures for cholesterol, triacylglycerols, total free fatty acids, and thiobarbituric acid reactive substances (TBARS) analysis. In addition, fatty acid methyl esters (FAMEs) were identified by gas chromatography.
Increased values of plasma corticosterone were found in RS and ELF-EMF exposed groups (p < 0.05), this effect was higher in RS + ELF-EMF group (p < 0.05, vs. control group). Chronic ELF-EMF exposure increased total lipids in cerebellum, and total cholesterol in cortex, but decreased polar lipids in cortex. In subcortical structures, increased concentrations of non-esterified fatty acids were observed in RS + ELF-EMF group. FAMEs analysis revealed a decrease of polyunsaturated fatty acids of cerebellum and increases of subcortical structures in the ELF-EMF exposed rats. TBARS concentration in lipids was increased in all treated groups compared to control group, particularly in cortex and cerebellum regions.
These findings suggest that chronic exposure to ELF-EMF is similar to physiological stress, and induce changes on brain lipid profile.
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